4OZ6

Structure of the Branched Intermediate in Protein Splicing

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-25
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97923
Spacegroup name	P 41 21 2
Unit cell lengths	58.746, 58.746, 111.762
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.489 - 2.786
R-factor	0.2314
Rwork	0.230
R-free	0.26170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1am2
RMSD bond length	0.003
RMSD bond angle	0.770
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.880
High resolution limit [Å]	2.780	2.780
Number of reflections	5310
<I/σ(I)>	39.6	9.7
Completeness [%]	99.7	96.4
Redundancy	8.7	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	Crystals were obtained by mixing 1 ul of protein with 1 ul of crystallization solution (100mM sodium cacodylate, pH 6.5, 200mM magnesium acetate, and 20% (w/v) PEG 8000.