4OZ1

Crystal structure of human CAPERalpha UHM bound to SF3b155 ULM5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	OTHER
Temperature [K]	100
Detector technology	CCD
Collection date	2013-01-03
Detector	APEX II CCD
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	44.653, 52.410, 85.141
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.989 - 1.740
R-factor	0.1472
Rwork	0.143
R-free	0.18050
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.010
RMSD bond angle	1.299
Data scaling software	PROTEUM PLUS
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.000	1.840
High resolution limit [Å]	1.700	1.740
Rmerge	0.070
Number of reflections	37595
<I/σ(I)>	19.1	3.2
Completeness [%]	97.4	90.2
Redundancy	6.9	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	0.8 M sodium phosphate, 0.8 M potassium phosphate, 0.1 M HEPES pH 7.5