4OZ0

Crystal structure of human CAPERalpha U2AF homology motif (apo-state)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	OTHER
Temperature [K]	100
Detector technology	CCD
Collection date	2013-01-03
Detector	APEX II CCD
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	44.653, 52.410, 85.141
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.571 - 2.200
R-factor	0.1985
Rwork	0.196
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3s6e
RMSD bond length	0.004
RMSD bond angle	0.894
Data scaling software	PROTEUM PLUS
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.650	2.290
High resolution limit [Å]	2.200	2.200
Rmerge	0.101	0.207
Number of reflections	17820
<I/σ(I)>	12.4	3.4
Completeness [%]	95.9	80.8
Redundancy	5.6	1.59

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1 M tri-sodium acetate pH 4.5, 0.1 M Bis-Tris pH 5.5, 25% (w/v) PEG 3350