4OYZ
Human solAC Complexed with Bicarbonate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97626 |
| Spacegroup name | P 63 |
| Unit cell lengths | 99.838, 99.838, 98.162 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.650 - 1.740 |
| R-factor | 0.169 |
| Rwork | 0.167 |
| R-free | 0.20310 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.100 |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 27.650 |
| High resolution limit [Å] | 1.740 |
| Number of reflections | 56027 |
| <I/σ(I)> | 14 |
| Completeness [%] | 98.5 |
| Redundancy | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 1ul of protein solution was mixed with 1ul of reservoir solution (0.1M sodium acetate, pH 4.8, 0.2M trisodium citrate, 16-18% PEG4K and 10% glycerol) and left to equilibrate at 4C |
