4OYY
Humicola insolens cutinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-06-20 |
| Detector | RIGAKU RAXIS II |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 125.548, 126.999, 134.505 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.761 - 3.000 |
| R-factor | 0.17481 |
| Rwork | 0.174 |
| R-free | 0.19597 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.445 |
| Data reduction software | MOSFLM |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.324 | |
| Number of reflections | 43738 | |
| <I/σ(I)> | 5.9 | 2.3 |
| Completeness [%] | 99.5 | 97.5 |
| Redundancy | 4.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | HiCutinase 30 mg mL-1, 0.1 M Tris/HCl buffer pH 8.5, 50 mM lysine, 100 mg mL-1 (app. 8.6%) isopropanol, 50 mg mL-1 (app. 4.3 %) dioxane, PEG 4K 22% v/v of 50% w/v stock solutions |
