4OYW
Crystal Structure of Human Soluble Adenylate Cyclase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-31 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97560 |
| Spacegroup name | P 63 |
| Unit cell lengths | 99.424, 99.424, 97.391 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.710 - 1.700 |
| R-factor | 0.1838 |
| Rwork | 0.182 |
| R-free | 0.21060 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.140 |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.700 |
| Number of reflections | 59877 |
| <I/σ(I)> | 9.4 |
| Completeness [%] | 99.8 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 1ul of protein solution was mixed with 1ul of reservoir solution (0.1M sodium acetate, pH 4.8, 0.2M trisodium citrate, 16-18% PEG4K and 10% glycerol) and left to equilibrate at 4C |
