4OY2

Crystal structure of TAF1-TAF7, a TFIID subcomplex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-11
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	122.215, 123.660, 229.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	83.676 - 2.900
R-factor	0.2105
Rwork	0.209
R-free	0.24330
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.806
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	84.000
High resolution limit [Å]	2.890
Rmerge	0.118
Number of reflections	77983
<I/σ(I)>	11.1
Completeness [%]	99.9
Redundancy	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		295	20% PEG3350, 150mM ammonium Citrate