4OXW

X-ray structure of a designed CISK-PX domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-25
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.978
Spacegroup name	P 21 21 21
Unit cell lengths	36.672, 49.258, 68.009
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.890 - 1.730
R-factor	0.19426
Rwork	0.193
R-free	0.21861
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xte
RMSD bond length	0.012
RMSD bond angle	1.497
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall
Low resolution limit [Å]	39.893
High resolution limit [Å]	1.730
Number of reflections	12689
<I/σ(I)>	57.75
Completeness [%]	99.2
Redundancy	12.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.2 M Ammonium Sulfate, 0.1 M Sodium Acetate pH 4.6, 30 %w/v Peg 2000 MME