4OXP

X-ray crystal structure of the S1 and 5'-sensor domains of RNase E from Caulobacter crescentus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-09-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9536
Spacegroup name	P 43 21 2
Unit cell lengths	62.530, 62.530, 155.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.700 - 2.100
R-factor	0.2138
Rwork	0.212
R-free	0.25434
RMSD bond length	0.019
RMSD bond angle	2.310
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall
Low resolution limit [Å]	48.700
High resolution limit [Å]	2.100
Rmerge	0.078
Number of reflections	18069
<I/σ(I)>	12.8
Completeness [%]	97.0
Redundancy	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Tris pH 8.5, 10% (v/v) Ethanol