4OXD
Structure of the LdcB LD-carboxypeptidase reveals the molecular basis of peptidoglycan recognition
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-11 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.916 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 345.954, 42.549, 79.318 |
| Unit cell angles | 90.00, 93.07, 90.00 |
Refinement procedure
| Resolution | 47.800 - 2.800 |
| R-factor | 0.276 |
| Rwork | 0.273 |
| R-free | 0.33400 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.842 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.800 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 47327 | |
| <I/σ(I)> | 7.8 | 1.9 |
| Completeness [%] | 98.3 | 98.3 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 0.2 M Ammonium chloride, 0.1 M Hepes pH 8, 20% PEG 6000 |
