4OX8

Structure of Prochlorococcus marinus str. MIT 9313 CsoS1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9762
Spacegroup name	P 1 21 1
Unit cell lengths	66.152, 58.553, 81.333
Unit cell angles	90.00, 113.33, 90.00

Refinement procedure

Resolution	37.340 - 1.903
R-factor	0.194
Rwork	0.192
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2a10
RMSD bond length	0.007
RMSD bond angle	1.076
Refinement software	PHENIX ((PHENIX.REFINE: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.340	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.111	0.800
Number of reflections	44660
<I/σ(I)>	11	1.86
Completeness [%]	99.2	95.5
Redundancy	3.7	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Sodium citrate pH 5.0 15% (w/v) PEG 8000