4OX0

Crystal structure of the keratin-like domain from the MADS transcription factor Sepallata 3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9393
Spacegroup name	P 21 21 2
Unit cell lengths	123.071, 143.197, 48.287
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.500 - 2.490
R-factor	0.2323
Rwork	0.230
R-free	0.27380
RMSD bond length	0.010
RMSD bond angle	1.250
Data scaling software	XDS
Refinement software	BUSTER-TNT (BUSTER 2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	60.000		2.550
High resolution limit [Å]	2.490	11.140	2.490
Rmerge	0.072	0.029	1.001
Rmeas	0.079	0.034	1.094
Total number of observations	177661
Number of reflections	30447	319	2252
<I/σ(I)>	13.99	34.45	1.8
Completeness [%]	98.9	77.2	99.3
Redundancy	5.8		6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	20-25% ethylene glycol