4OVM

Crystal structure of SgcJ protein from Streptomyces carzinostaticus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-08-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97935
Spacegroup name	P 1 21 1
Unit cell lengths	98.318, 52.827, 131.796
Unit cell angles	90.00, 90.14, 90.00

Refinement procedure

Resolution	37.992 - 2.719
R-factor	0.2189
Rwork	0.216
R-free	0.25620
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.648
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	HKL-3000
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.770
High resolution limit [Å]	2.720	7.380	2.720
Rmerge	0.084	0.046	0.959
Total number of observations	262129
Number of reflections	36879
<I/σ(I)>	11.5		2.71
Completeness [%]	99.8	95.4	100
Redundancy	7.1	6.6	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.2 M Sodium Formate, 20% w/v PEG 3350