4OVM
Crystal structure of SgcJ protein from Streptomyces carzinostaticus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97935 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 98.318, 52.827, 131.796 |
| Unit cell angles | 90.00, 90.14, 90.00 |
Refinement procedure
| Resolution | 37.992 - 2.719 |
| R-factor | 0.2189 |
| Rwork | 0.216 |
| R-free | 0.25620 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.648 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.770 |
| High resolution limit [Å] | 2.720 | 7.380 | 2.720 |
| Rmerge | 0.084 | 0.046 | 0.959 |
| Total number of observations | 262129 | ||
| Number of reflections | 36879 | ||
| <I/σ(I)> | 11.5 | 2.71 | |
| Completeness [%] | 99.8 | 95.4 | 100 |
| Redundancy | 7.1 | 6.6 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2 M Sodium Formate, 20% w/v PEG 3350 |
