4OVE

X-ray Crystal Structure of Mouse Netrin-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-14
Detector	RAYONIX MX-300
Wavelength(s)	1.25440
Spacegroup name	P 32 2 1
Unit cell lengths	69.754, 69.754, 334.802
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.640
R-factor	0.233
Rwork	0.230
R-free	0.28800
Structure solution method	SIRAS
RMSD bond length	0.017
RMSD bond angle	1.806
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.500
Rmerge	0.129
Number of reflections	27279
<I/σ(I)>	8.8
Completeness [%]	80.9
Redundancy	10.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.7	293	0.1 M HEPES, 2.7-2.8 M NaCl, 0.02-0.2 M glycine, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7.7	293	0.1 M HEPES, 2.7-2.8 M NaCl, 0.02-0.2 M glycine, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K