4OUS
Crystal structure of zebrafish Caprin-2 C1q domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-23 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97922 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 94.985, 94.985, 94.985 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.620 - 1.050 |
| R-factor | 0.1523 |
| Rwork | 0.152 |
| R-free | 0.16130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o91 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.127 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.090 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Number of reflections | 63149 | |
| <I/σ(I)> | 17.9 | 3.3 |
| Completeness [%] | 95.2 | 100 |
| Redundancy | 7.1 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 291 | 4.0M sodium nitrate, 0.1M Bis-Tris propane pH7.0, VAPOR DIFFUSION, temperature 291K |
