4OUN
Crystal Structure of Mini-ribonuclease 3 from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.917152 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 41.092, 62.028, 89.388 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.690 - 1.800 |
| R-factor | 0.2092 |
| Rwork | 0.206 |
| R-free | 0.26760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u61 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.542 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.5.0) |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 44.700 | 44.690 | 1.910 |
| High resolution limit [Å] | 1.800 | 5.360 | 1.800 |
| Rmerge | 0.091 | 1.400 | |
| Rmeas | 0.040 | 1.468 | |
| Number of reflections | 10939 | 461 | 1732 |
| <I/σ(I)> | 17.17 | 58.11 | 2.04 |
| Completeness [%] | 99.8 | 100 | 99.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.2M sodium acetate trihydrate, 0.1M TRIS, 30% PEG4000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
