4OUJ
Crystal structure of HA33B-Lac
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.819, 36.959, 114.624 |
| Unit cell angles | 90.00, 102.11, 90.00 |
Refinement procedure
| Resolution | 19.801 - 1.460 |
| R-factor | 0.1635 |
| Rwork | 0.163 |
| R-free | 0.18070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lo2 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.209 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.053 | 1.540 |
| High resolution limit [Å] | 1.460 | 1.460 |
| Rmerge | 0.040 | 0.413 |
| Number of reflections | 119409 | |
| <I/σ(I)> | 15.9 | 2.3 |
| Completeness [%] | 99.0 | 97.1 |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 0.1 M Hepes, 5% MPD, 5% (w/v) PEG 6000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
