4OTX
Structure of the anti-Francisella tularensis O-antigen antibody N203 Fab fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-09-17 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.011, 122.322, 124.764 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.857 - 2.100 |
R-factor | 0.194 |
Rwork | 0.193 |
R-free | 0.22830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | the light chain of PDB code 1MCP and the heavy (H) chain of PDB code 3CLE |
RMSD bond length | 0.002 |
RMSD bond angle | 0.674 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.5.0) |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 15.000 | 15.000 | 2.170 |
High resolution limit [Å] | 2.100 | 4.490 | 2.100 |
Rmerge | 0.070 | 0.035 | 0.349 |
Number of reflections | 58890 | ||
<I/σ(I)> | 14.4 | ||
Completeness [%] | 99.0 | 97.8 | 96.3 |
Redundancy | 4.1 | 4.1 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 290 | Crystals were grown by streak seeding into an overnight equilibrated drop containing 0.5 ul of Fab (38mg/ml) and 0.5 ul of reservoir solution [0.1 M sodium citrate (pH 4.0), 1 M lithium chloride, 13-16% PEG 6000], VAPOR DIFFUSION, HANGING DROP, temperature 290K |