4OTT

Crystal structure of the gamma-glutamyltranspeptidase from Bacillus licheniformis.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2013-01-29
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	60.896, 61.968, 148.245
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.250 - 2.980
R-factor	0.19442
Rwork	0.191
R-free	0.25040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB code 3A75
RMSD bond length	0.011
RMSD bond angle	1.686
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.950
Number of reflections	11668

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.2	298	Protein concentration 20 mg/ml, 20% PEG3350, 0.2 M MgCl2, 0.2 M Tris-HCl, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K