4OTN

Crystal structure of the C-terminal regulatory domain of murine GCN2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97952
Spacegroup name	P 32 2 1
Unit cell lengths	85.494, 85.494, 73.041
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.021 - 1.900
R-factor	0.1974
Rwork	0.195
R-free	0.23620
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.104
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	HKL-3000
Refinement software	PHENIX (1.4_4)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.930
High resolution limit [Å]	1.900	5.160	1.900
Rmerge	0.045	0.024	0.641
Number of reflections	24605
<I/σ(I)>	15.8
Completeness [%]	99.6	94.3	99.3
Redundancy	6.6	6	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	298	0.1 M Bis-Tris, 2.0 M ammonium sulfate, pH 6.5, VAPOR DIFFUSION, temperature 298K