4OT7
X-structure of a variant of NCR from zymomonas mobilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-11 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 121.240, 121.240, 58.650 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.700 - 1.800 |
| R-factor | 0.17044 |
| Rwork | 0.170 |
| R-free | 0.19553 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4a3u |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.051 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 39.700 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.068 |
| Number of reflections | 46089 |
| <I/σ(I)> | 22.49 |
| Completeness [%] | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 292 | 0.1 M sodium citrate, 1.0 M lithium chloride, 21% PEG 6000, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
