4OSH
Crystal structure of the TAL effector dHax3 with NI RVD at 2.2 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0092 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.529, 80.987, 89.083 |
| Unit cell angles | 90.00, 103.62, 90.00 |
Refinement procedure
| Resolution | 36.633 - 2.201 |
| R-factor | 0.207 |
| Rwork | 0.205 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3v6t |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.289 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CCP4 |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.770 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.610 | 2.200 |
| Rmerge | 0.242 | 0.588 | |
| Number of reflections | 58921 | ||
| <I/σ(I)> | 7.4 | 2.4 | |
| Completeness [%] | 99.9 | 99.7 | 90.5 |
| Redundancy | 3.6 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 10%-15% PEG 3350, 12% ethanol, 0.1M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
