4OS0
Three-dimensional structure of the C65A-W54F double mutant of Human lipocalin-type Prostaglandin D Synthase apo-form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-01-30 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 |
Unit cell lengths | 41.622, 45.870, 48.260 |
Unit cell angles | 69.40, 77.67, 65.32 |
Refinement procedure
Resolution | 45.036 - 1.750 |
R-factor | 0.1978 |
Rwork | 0.196 |
R-free | 0.23280 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.004 |
RMSD bond angle | 0.944 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.040 | 1.850 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.067 | 0.352 |
Number of reflections | 29286 | |
<I/σ(I)> | 10 | 3.4 |
Completeness [%] | 95.9 | 95 |
Redundancy | 3.6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 02.M Na/K Tartrate, 0.1M Na acetate, 2.0M Ammonium sulphate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |