4ORO
Structure of Influenza B PB2 cap-binding domain complex with GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-01-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 83.420, 42.771, 118.414 |
| Unit cell angles | 90.00, 90.08, 90.00 |
Refinement procedure
| Resolution | 39.470 - 2.400 |
| R-factor | 0.20271 |
| Rwork | 0.199 |
| R-free | 0.26982 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4or4 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.987 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 32029 | |
| Completeness [%] | 96.6 | 96.3 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 293 | 20% PEG 3350, 0.2M potassium sodium tartrate, 0.1M Bis-Tris, pH 6.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
