4OR6

Structure of Influenza B PB2 cap-binding domain with Q325F mutation complex with GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	42.974, 90.151, 95.706
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.134 - 2.293
R-factor	0.1878
Rwork	0.185
R-free	0.24570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4or4
RMSD bond length	0.002
RMSD bond angle	0.614
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.360
High resolution limit [Å]	2.290	2.290
Number of reflections	17179
Completeness [%]	99.9	99.9
Redundancy	9.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	20% PEG 3350, 0.15M DL-Malic acid, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K