4OR4

Structure of Influenza B PB2 cap-binding domain with Q325F mutation complex with m7GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	42.936, 90.698, 95.774
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.350 - 2.210
R-factor	0.17928
Rwork	0.176
R-free	0.24194
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4eqk
RMSD bond length	0.022
RMSD bond angle	2.213
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.240
High resolution limit [Å]	2.200	2.200
Number of reflections	19358
Completeness [%]	99.9	100
Redundancy	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	25% PEG 3350, 0.2M ammonium acetate, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K