4OR1
Structure and mechanism of fibronectin binding and biofilm formation of enteroaggregative Escherischia coli AAF fimbriae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-10-02 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9778 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 81.755, 81.755, 222.703 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 51.240 - 3.000 |
| R-factor | 0.22149 |
| Rwork | 0.220 |
| R-free | 0.24840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2axw |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.181 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0062) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.240 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 9081 | |
| <I/σ(I)> | 6.5 | 2.6 |
| Completeness [%] | 97.0 | 98.2 |
| Redundancy | 2.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1 M BIS-TRIS, 0.2 M LiSO4, 25% PEG3350., pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
