4OQY
Streptomyces sp. GF3546 imine reductase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.320, 68.240, 181.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.530 - 1.900 |
| R-factor | 0.2177 |
| Rwork | 0.216 |
| R-free | 0.24129 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.498 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 54.590 |
| High resolution limit [Å] | 1.900 |
| Rmerge | 0.094 |
| Number of reflections | 44024 |
| <I/σ(I)> | 6.9 |
| Completeness [%] | 97.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 278 | 2.2 M (NH4)2SO4, 0.1 M BisTris, microseeding, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 278K |
