4OQ2
5hmC specific restriction endonuclease PvuRTs1I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-06-27 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97630 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 61.990, 61.990, 210.990 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.830 - 2.350 |
R-factor | 0.20563 |
Rwork | 0.203 |
R-free | 0.25397 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.293 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELXDE |
Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.410 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.048 | 0.970 |
Number of reflections | 17509 | |
<I/σ(I)> | 19.5 | 2.2 |
Completeness [%] | 96.6 | 99 |
Redundancy | 5.7 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 10% w/v PEG4000, 20% v/v glycerol, 20 mM D-glucose, 20 mM D-mannose, 20 mM D-galactose, 20 mM L-fucose, 20 mM D-xylose, 20 mM N-acetyl-D-glucosamine, 0.1 M MOPS/HEPES sodium, pH 7.5, for cryoprotection, crystals were transferred to a variant of the buffer with 28% v/v glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 277K |