4OPV
Unliganded crystal structure of P domain from norovirus strain Farmington Hills 2004 co-crystallized with HBGA type Lea
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.93223 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.130, 90.150, 109.480 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.758 - 1.850 |
| R-factor | 0.1495 |
| Rwork | 0.148 |
| R-free | 0.17490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.078 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.5.3) |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.900 | |
| High resolution limit [Å] | 1.850 | 8.270 | 1.850 |
| Rmerge | 0.062 | 0.013 | 0.420 |
| Number of reflections | 53177 | ||
| <I/σ(I)> | 20.94 | 62.72 | 3.52 |
| Completeness [%] | 98.4 | 97.8 | 86.2 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | PEG 3350, Magnesium formate, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
