4OPN
Crystal structure of mouse glyoxalase I complexed with mAH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-15 |
| Detector | OXFORD ONYX CCD |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.559, 65.084, 64.885 |
| Unit cell angles | 90.00, 101.29, 90.00 |
Refinement procedure
| Resolution | 25.615 - 2.100 |
| R-factor | 0.1798 |
| Rwork | 0.177 |
| R-free | 0.23120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.011 |
| Data reduction software | CrysalisPro |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 25.615 | 25.617 | 2.211 |
| High resolution limit [Å] | 2.100 | 2.211 | 2.100 |
| Number of reflections | 19556 | ||
| Completeness [%] | 98.2 | 98.2 | 95.92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 289 | 30% PEG 2000, 50mM MES, 0.1M NaCl, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
