4OPK
Bh-RNaseH:2'-SMe-DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-21 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 98.138, 66.609, 77.898 |
| Unit cell angles | 90.00, 122.05, 90.00 |
Refinement procedure
| Resolution | 66.020 - 1.539 |
| R-factor | 0.19893 |
| Rwork | 0.194 |
| R-free | 0.25820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3d0p |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.324 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.024 | 1.570 |
| High resolution limit [Å] | 1.539 | 1.539 |
| Rmerge | 0.047 | 0.675 |
| Number of reflections | 59362 | |
| <I/σ(I)> | 28.3 | 1.27 |
| Completeness [%] | 94.2 | 91.8 |
| Redundancy | 2.4 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 291 | 0.1 M sodium acetate, 8% w/v PEG4000, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
