4OPJ
Bh-RNaseH:tcdA-DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-21 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.387, 47.501, 55.216 |
| Unit cell angles | 100.87, 101.77, 89.75 |
Refinement procedure
| Resolution | 53.050 - 1.541 |
| R-factor | 0.1668 |
| Rwork | 0.162 |
| R-free | 0.22007 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3d0p |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.054 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.054 | 1.650 |
| High resolution limit [Å] | 1.541 | 1.541 |
| Rmerge | 0.048 | |
| Number of reflections | 56542 | |
| <I/σ(I)> | 19.52 | 4.4 |
| Completeness [%] | 92.5 | 86.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 291 | 0.1 M sodium acetate, 10% w/v PEG4000, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
