4OPE
Streptomcyes albus JA3453 oxazolomycin ketosynthase domain OzmH KS7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 135.312, 139.834, 173.786 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.075 - 2.580 |
| R-factor | 0.17978 |
| Rwork | 0.177 |
| R-free | 0.23061 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.496 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 41.100 | 50.000 | 2.630 |
| High resolution limit [Å] | 2.590 | 7.020 | 2.590 |
| Rmerge | 0.150 | 0.074 | 0.909 |
| Number of reflections | 103438 | ||
| <I/σ(I)> | 8.7 | 2.5 | |
| Completeness [%] | 99.9 | 98.9 | 100 |
| Redundancy | 7.3 | 6.7 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 297 | 0.2 M sodium nitrate, 20% PEG 3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
