4OP9
Urate OXIDASE IN COMPLEX WITH 8-AZAXANTHINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM1A |
| Synchrotron site | ESRF |
| Beamline | BM1A |
| Temperature [K] | 291 |
| Detector technology | CCD |
| Collection date | 2012-06-22 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.945 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 80.109, 96.175, 105.407 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.580 |
| R-factor | 0.16897 |
| Rwork | 0.168 |
| R-free | 0.19055 |
| Structure solution method | RIGID BODY |
| Starting model (for MR) | 2iba |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.342 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 1.610 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.042 | 0.364 |
| Number of reflections | 55375 | |
| <I/σ(I)> | 33.4 | 2.9 |
| Completeness [%] | 98.8 | 95.6 |
| Redundancy | 5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH | 8.5 | 291 | 10-15MG/ML URATE OXIDASE, 8-AZAXANTHIN IN EXCESS, 5-8% PEG8000, 50MM TRIS ACETATE, pH 8.5, BATCH, temperature 291K |
