4OP7
Crystal structure of P domain from norovirus strain NSW0514 in complex with HBGA type B (triglycan)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.978174 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 104.610, 104.610, 190.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.187 - 1.920 |
| R-factor | 0.1859 |
| Rwork | 0.185 |
| R-free | 0.20810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.102 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.5.3) |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.190 | 1.950 | |
| High resolution limit [Å] | 1.900 | 8.500 | 1.900 |
| Rmerge | 0.113 | 0.012 | 0.012 |
| Number of reflections | 78721 | ||
| <I/σ(I)> | 10.7 | 45.1 | 1.09 |
| Completeness [%] | 93.8 | 77.9 | 93.7 |
| Redundancy | 4.3 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | PEG 3350, Sodium formate, vapor diffusion, hanging drop, temperature 291K |
