4OOX
Crystal structure of P domain from norovirus strain Saga4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-28 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97243 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 96.720, 58.940, 62.140 |
| Unit cell angles | 90.00, 119.88, 90.00 |
Refinement procedure
| Resolution | 27.173 - 1.200 |
| R-factor | 0.1208 |
| Rwork | 0.120 |
| R-free | 0.14320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.363 |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.5.3) |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 96.029 | 1.060 | |
| High resolution limit [Å] | 1.030 | 4.610 | 1.030 |
| Rmerge | 0.034 | 0.029 | 0.548 |
| Number of reflections | 135999 | 1686 | 9760 |
| <I/σ(I)> | 15.46 | 49.1 | 1.8 |
| Completeness [%] | 95.8 | 95.7 | 89.4 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 291 | Sodium acetate, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
