4OOS
Crystal structure of P domain from norovirus strain NSW0514
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.978174 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 98.480, 55.070, 63.460 |
| Unit cell angles | 90.00, 120.10, 90.00 |
Refinement procedure
| Resolution | 42.599 - 1.640 |
| R-factor | 0.1389 |
| Rwork | 0.138 |
| R-free | 0.16150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.090 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.5.2) |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 1.640 | ||
| High resolution limit [Å] | 1.600 | 7.160 | 1.600 |
| Rmerge | 0.044 | 0.017 | 0.247 |
| Number of reflections | 38171 | 441 | 2706 |
| <I/σ(I)> | 20.84 | 46.76 | 5.04 |
| Completeness [%] | 97.8 | 93.6 | 94 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | PEG 3350, Sodium formate, vapor diffusion, hanging drop, temperature 291K |
