4OOD
Structure of K42Y mutant of sperm whale myoglobin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.002, 47.515, 85.243 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.390 - 1.240 |
| R-factor | 0.14318 |
| Rwork | 0.140 |
| R-free | 0.19468 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3OCK |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.430 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 1.271 | |
| High resolution limit [Å] | 1.240 | 1.240 |
| Number of reflections | 36894 | |
| Completeness [%] | 94.3 | 65.15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 2.7 M ammonium sulfate, 20 mM Tris/HCl, 1 mM EDTA, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
