4OO6

Crystal structure of human KAP-beta2 bound to the NLS of HCC1 (Hepato Cellular Carcinoma protein 1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0750
Spacegroup name	P 21 21 2
Unit cell lengths	126.584, 162.152, 68.501
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.700
R-factor	0.1912
Rwork	0.189
R-free	0.23840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fdd
RMSD bond length	0.011
RMSD bond angle	1.440
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.810
High resolution limit [Å]	2.700	2.700
Rmerge	0.102	1.295
Number of reflections	39520
<I/σ(I)>	13.3	1.4
Completeness [%]	99.9	99.7
Redundancy	5.3	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.25	298	"Protein (20 MM HEPES, PH 7.3, 110 MM POTASSIUM ACETATE, 2MM MAGNESIUM ACET ATE, 20% GLYCEROL, 2MM DTT); Reservoir (0.2 M Sodiumchloride, 0.8M Sodiumcitrate, 0.1M BisTris pH 6.25 ); Cryo (2.5 M Sodiummalonate)">, VAPOR DIFFUSION, SITTING DROP, temperature 298K