4ONH
Crystal Structure of DN6 TCR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-11-20 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.03321 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 143.825, 64.063, 69.534 |
| Unit cell angles | 90.00, 115.88, 90.00 |
Refinement procedure
| Resolution | 24.674 - 3.008 |
| R-factor | 0.2598 |
| Rwork | 0.257 |
| R-free | 0.31650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.920 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | PHENIX (1.7_650) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 8.130 | 3.000 |
| Rmerge | 0.122 | 0.107 | 0.561 |
| Number of reflections | 11289 | ||
| <I/σ(I)> | 18.5 | ||
| Completeness [%] | 98.8 | 92.7 | 93.4 |
| Redundancy | 3.8 | 3.7 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | 0.1 M Mes, pH-6.5, 20% PEG 4000, 0.6 M NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
