4OMZ

Crystal Structure of NolR from Sinorhizobium fredii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	99.771, 99.619, 113.017
Unit cell angles	90.00, 90.08, 90.00

Refinement procedure

Resolution	37.400 - 2.640
R-factor	0.205
Rwork	0.203
R-free	0.24600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.105
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	SHELXS
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	37.400
High resolution limit [Å]	2.640
Number of reflections	32349
<I/σ(I)>	17.7
Completeness [%]	99.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.2	277	1.6 M sodium phosphate monobasic/0.4 M potassium phosphate dibasic, 0.1 M sodium phosphate dibasic/citric acid, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K