4OMV

Crystal Structure of a Putative Macrophage Growth Locus, subunit A From Francisella tularensis SCHU S4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2012-03-20
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97857
Spacegroup name	P 61
Unit cell lengths	104.977, 104.977, 101.659
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.510 - 2.750
R-factor	0.1904
Rwork	0.187
R-free	0.22470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3mdk
RMSD bond length	0.010
RMSD bond angle	1.110
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	BUSTER-TNT

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.800
High resolution limit [Å]	2.750	7.460	2.750
Rmerge	0.075	0.049	0.748
Number of reflections	16516
<I/σ(I)>	12.4
Completeness [%]	99.1	97.8	99.6
Redundancy	4.8	4.3	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	NaFormate 4M, Tacsimate 3%, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K