4OMU
Crystal structure of shikimate dehydrogenase (AroE) from Pseudomonas putida
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2009-08-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97948 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.555, 84.393, 94.404 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.200 - 1.650 |
| Rwork | 0.181 |
| R-free | 0.21680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p77 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.115 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 47.200 |
| High resolution limit [Å] | 1.650 |
| Rmerge | 0.056 |
| Number of reflections | 64531 |
| <I/σ(I)> | 33.4 |
| Completeness [%] | 98.2 |
| Redundancy | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 298 | 0.1M NaHEPES, 2M Ammonium Sulfate, 2% PEG400, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
