4OMT
Crystal structure of human muscle phosphofructokinase (dissociated homodimer)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-02 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 229.700, 229.700, 133.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.950 - 6.000 |
| R-factor | 0.2417 |
| Rwork | 0.238 |
| R-free | 0.27390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3o8n |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.446 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.950 | 6.710 |
| High resolution limit [Å] | 6.000 | 6.000 |
| Rmerge | 0.106 | |
| Number of reflections | 5537 | |
| <I/σ(I)> | 12.5 | 4.7 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 11.6 | 12.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 292 | 0.6 M LiNO3, 0.05 M NaF, 0.1 M sodium acetate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
