4OMB
Phosphate binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-31 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9508 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.444, 148.092, 216.452 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 122.200 - 1.500 |
| R-factor | 0.19144 |
| Rwork | 0.190 |
| R-free | 0.21571 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1twy |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.639 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 122.220 | 2.590 | 1.590 |
| High resolution limit [Å] | 1.490 | 2.250 | 1.490 |
| Rmerge | 0.147 | ||
| Number of reflections | 186078 | ||
| <I/σ(I)> | 11.89 | ||
| Completeness [%] | 98.0 | 100 | 91 |
| Redundancy | 13.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 293 | 2.5 M Na Malonate, 0.1 M Tris, pH 8.0, VAPOR DIFFUSION, temperature 293K |
