4OM7
Crystal structure of TIR domain of TLR6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2013-07-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 127.607, 44.199, 75.723 |
| Unit cell angles | 90.00, 118.90, 90.00 |
Refinement procedure
| Resolution | 31.822 - 2.204 |
| R-factor | 0.236 |
| Rwork | 0.233 |
| R-free | 0.28530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.390 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.400 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 18245 | |
| Completeness [%] | 14.0 | 92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 298 | 0.2M lithium sulfate monohydrate, 0.1M Tris pH 8.6, 25%(w/v) polyethylene glycol 3350 and 4%(v/v) formamide, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
