4OLT
Chitosanase complex structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.023, 40.705, 104.794 |
| Unit cell angles | 90.00, 106.53, 90.00 |
Refinement procedure
| Resolution | 33.900 - 1.590 |
| R-factor | 0.164 |
| Rwork | 0.161 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1chk |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.814 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.900 | 1.640 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.250 | |
| Number of reflections | 63744 | |
| <I/σ(I)> | 12.7 | 1.6 |
| Completeness [%] | 99.4 | 99.7 |
| Redundancy | 6.5 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 288 | 0.05 M KH2PO4, 23% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
