4OLR
[Leu-5]-Enkephalin mutant - YVVFV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 9.750, 21.380, 19.050 |
| Unit cell angles | 90.00, 93.17, 90.00 |
Refinement procedure
| Resolution | 19.020 - 1.100 |
| R-factor | 0.1532 |
| Rwork | 0.150 |
| R-free | 0.18710 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.376 |
| Data scaling software | XSCALE |
| Phasing software | SHELXD |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 19.020 | 1.130 | |
| High resolution limit [Å] | 1.100 | 4.920 | 1.100 |
| Rmerge | 0.212 | 0.095 | 0.769 |
| Number of reflections | 3191 | 38 | 203 |
| <I/σ(I)> | 5.91 | 14.08 | 1.5 |
| Completeness [%] | 98.4 | 97.4 | 90.2 |
| Redundancy | 6.65 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | reservoir contained 15% MPD, 0.1M Sodium Cacodylate, 0.1M Magnesium Acetate, pH 6.5, vapor diffusion, hanging drop, temperature 298K |
