4OLL
cAMP-binding acyltransferase from Mycobacterium smegmatis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-07-15 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.97500 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.305, 82.750, 105.514 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.110 - 1.900 |
| R-factor | 0.2119 |
| Rwork | 0.206 |
| R-free | 0.26260 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.851 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 65.110 | 50.000 | 1.940 |
| High resolution limit [Å] | 1.900 | 4.680 | 1.900 |
| Rmerge | 0.102 | 0.088 | 0.668 |
| Number of reflections | 27052 | ||
| <I/σ(I)> | 9.7 | ||
| Completeness [%] | 99.3 | 99 | 98.4 |
| Redundancy | 12.8 | 12.2 | 11.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG 3350, NaCl, Bis-Tris, CaCl2, DAPD, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
